The project ProTecteDNA focuses on biodiversity data acquisition from environmental DNA (eDNA). eDNA consists of molecules that are present in the environment in different forms, from DNA fragments to cells, hair, scales, tissue particles or whole organisms. The detection of eDNA and species determination can be done in an efficient, scalable and affordable way and thus promote successful biodiversity monitoring. Due to technology advances over the last two decades, the use of genetic methods in biodiversity assessment has become widespread and often already passed experimental and demonstrative level of technology readiness. The metabarcoding of diverse taxa requires dedicated facilities and expensive equipment, such as certified laboratories and sequencing devices, respectively. However, new technologies have given rise to novel innovative approaches that make DNA-based methods affordable and applicable for biodiversity monitoring without major infrastructure and research teams of molecular biologists. In the project, a portable laboratory will be tested for its implementation of biodiversity assessment in freshwater habitats of protected areas.